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Renat Krylov
Renat Krylov

Squid Developer



Molluscs represent a third phylum in which highly complex eyes are present. Cephalopod molluscs (squid, octopus, cuttlefish) possess a well developed nervous system and are highly intelligent (17). The complex eyes of cephalopod molluscs and vertebrates have been considered a classical example of convergent evolution (18). The eyes in these two systematic groups are remarkably similar in general appearance and organization but they are formed by different mechanisms during development and differ in many details.




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(A) Nucleotide and deduced amino acid sequences of the squid L. opalescens embryonic Pax-6 cDNA. The paired domain and homeodomain are boxed. Two arrows indicate the positions of two known introns in the L. opalescens Pax-6 gene. (B) Comparison of the amino acid sequences between paired-, homeo- and C-terminal domains of vertebrate and invertebrate species. The squid sequence is shown in full; for other sequences only differing amino acids are shown. A - indicates gaps that were introduced to maximize similarities in the C-terminal domain; an * marks the end of the known nemertine sequence. The percent identities are shown for the squid sequence.


It is not clear at present whether squid Pax-6 initiates the development of ectopic eyes in Drosophila directly or indirectly by activating eyeless. Moreover, it is not known how many common genes acting downstream of Pax-6 are involved in the cascade leading to eye development in different systematic groups. At least two other candidate genes have been identified. One is eyes absent which is necessary for Drosophila eye development (51); two homologous genes for eyes absent are expressed in vertebrate lens and retina (52), and one homolog was recently identified in the squid (S.I.T., unpublished data). The homeobox gene, sine oculis, is also essential for eye development in Drosophila (53) and acts after eyes absent (54). The sine oculis homolog, Six-3, is expressed in the vertebrate eye (55). sine oculis homologs have not been identified yet in the squid.


We thank Dr. J. Marthy for help with the isolation of squid spermatophores for DNA isolation and Drs. W. Gilly and M. Perri for help with collection and fixing of squid embryos. We thank Drs. W. Gilly, P. Grant, and J. West for help with analysis of the results of in situ hybridization; A. Cvekl for pointing out the potential Pax-6 binding sites in S-crystallin promoters, and Frederick Biomedical Supercomputing Center, Frederick Cancer Research and Development Center (Frederick, MD) for allocation of computing time and staff support. This work has been supported by grants from the Swiss National Science Foundation and the Kantons of Basel To W.G., from the Janggen-Pöhn Stiftung to G.H., and from the Collen Foundation and the Sandoz Foundation to P.C.


A staging series based on easily distinguishable morphological features is a basic and necessary tool for developmental studies. It provides a consistent reference for comparisons between independent studies, negates the need to know when fertilization occurred, allows correlation of the phase of development with the time of development (to facilitate collection of embryos at specific stages), and allows comparisons between species. Given the growing interest in Hawaiian bobtail squid (Euprymna scolopes) as a contemporary cephalopod developmental system, this article provides a detailed survey of E. scolopes embryogenesis from cleavage through hatching under controlled environmental conditions, including detailed descriptions of externally visible morphological features that are easily distinguished in either live or freshly fixed embryos under a dissecting microscope. Photomicrographs are also provided to aid in the accurate and rapid staging of E. scolopes embryos.


Developmental biology is among the many subdisciplines of the life sciences being transformed by our increasing awareness of the role of coevolved microbial symbionts in health and disease. Most symbioses are horizontally acquired, i.e., they begin anew each generation. In such associations, the embryonic period prepares the animal to engage with the coevolved partner(s) with fidelity following birth or hatching. Once interactions are underway, the microbial partners drive maturation of tissues that are either directly associated with or distant from the symbiont populations. Animal alliances often involve complex microbial communities, such as those in the vertebrate gastrointestinal tract. A series of simpler-model systems is providing insight into the basic rules and principles that govern the establishment and maintenance of stable animal-microbe partnerships. This review focuses on what biologists have learned about the developmental trajectory of horizontally acquired symbioses through the study of the binary squid-vibrio model.


It is possible to rely on the images we use for it to test code changesagainst different Linux distributions and compiler versions. We publishthese images to theDocker Hub. They arenamed squidcache/buildfarm--


The set of new features is determined by submissions and availabledeveloper time. New features may be completed and added at any time.Features accepted before 2023-02-05 (see ReleaseSchedule)will be part of this release.


The developers said the token would officially launch in November as the exclusive coin of a Squid Game-themed play-to-earn platform. Participants could enter the online tournament through an entry fee and promoters said winners would be rewarded with more SQUID tokens.


The discovery, made using high-resolution cameras focused on the retinas of longfin squid (Doryteuthis pealeii) embryos, reveals that, in spite of 500 million years of divergent evolution, the basic blueprint for how complex brains and nervous systems evolve may be the same across a wide range of species.


This is not the only time that scientists have spotted cephaolopods sharing common neurological blueprints with us. Much like humans, octopuses and squid also have a large variety of microRNAs (small molecules that control how genes are expressed) found inside their neural tissue.


Next, the team wants to look at how and when different cell types in the squid emerge as tissue grows and compare this process to the one observed in vertebrate embryos. If the blueprint for growth is the same, then perhaps the timetable could be, as well.


There has been such a considerable expansion of our knowledge of squid embryology since the first edition of this book was written that a complete review of the literature is impossible. The scientific importance and the commercial value of squids have prompted an increased interest in all aspects of squid biology; embryology has followed this trend. The reader is particularly referred to the reviews of Arnold (1974), Arnold and Williams-Arnold (1976), Fioroni and Meister (1974), Boletzky (1987, 1988), Segmüller, M. and Marthy (1989), and the comments of Marthy (1982) for an idea of the status of experimental investigations of the cephalopods and theories on the regulation of ontogeny, as well as some of the outstanding questions concerning development. What follows here is a brief description of the embryonic development of the squid; no survey of the vast literature is attempted. See Arnold (1974) for a fairly extensive list of references for major sources of embryonic information.


Investors ignored all these red flags and poured money into the project. According to BscScan, the developers exchanged the token for Binance Coin, making off with $3.35 million. They then used coin mixer Tornado Cash to obfuscate their moves and have since then vanished.


Rob Hale, the indie game developer best known as Squid in a Box, has passed away. Earlier this month, Hale died of cancer, and his partner CJ recently broke the news on the Steam forums of Hale's game, Waves 2: Notorious.


Prior to indie development, Hale had worked as a modder and developer on triple-A titles. Their first solo game, Waves, was a twin-stick shooter that released in 2011. Waves 2 released as an Early Access title in 2015 and will remain unfinished. Despite that, CJ pointed out that Rob was "dedicated to their craft," even when that ambition didn't always lead to success.


Start the development by defining the data schema in schema.graphql in the squid root folder. The schema will be used both for the target database and for the GraphQL API. It consists of regular GraphQL type declarations annotated with custom directives to define:


The squid processor data handlers use TypeORM entitiesto interact with the target database during the data processing. All necessary entity classes aregenerated by the squid framework from schema.graphql with


A squid processor is a node.js process that fetches historical on-chain data, performs arbitrary transformations and saves the result into the target database schema defined above. By convention, the processor entry point is src/processor.ts.


When squid eggs hatch, the hatchlings, or paralarvae, become part of the plankton; learning how they behave will help us learn where they go post-hatching and to develop a broader understanding of the importance of inshore spawning grounds to the species.


Riju Chakraborty will be doing a Hands On Workshop on Squid. Skuid is an app exchange product built using javascript. It enables developer to create UI with point and click configuration without writing apex code. The tool also provides flexibility to enhance out of the box components and create new components. This product hits a very good balance between custom vs out of the box.


This will store your username along with a hash of your new password in /etc/squid/passwords, which will be used as an authentication source by Squid. You can cat the file afterward to see what that looks like:


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